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Title :相同染色体近傍領域間相互作用によるイネ雑種弱勢の原因遺伝子単離と解析
Authors :久保山, 勉
Authors alternative :Kuboyama, Tsutomu
Issue Date :26-Jun-2018
Abstract :研究成果の概要(和文):岡らによって報告されたイネ雑種弱勢は第11染色体のほぼ相同な領域に座乗するHwa1-1とHwa2-1によって生じる.本課題はこれらの遺伝子の単離を目的として実施された.長鎖の配列を出力する次世代シーケンサーを用いた塩基配列解読結果を高密度連鎖解析によって絞り込まれた位置情報に基づき解析し、HWA1領域の配列の連なり(contig)を構築することができた.Hwa1-1領域は、日本晴と比較して20kbpの欠失が認められた.HWA2については1つのcontigにまとめることができず、3つのcontigに分かれた.Hwa2-1の領域は日本晴のゲノム配列とは構造が大きく異なっていることが明らかになった. 研究成果の概要(英文):Oka reported that epistatic interaction between Hwa1-1and Hwa2-1 cause a hybrid weakness of rice and we have shown that these causal genes located on the almost the same region of chromosome 11. This research project aimed at molecular cloning of Hwa1-1and Hwa2-1. DNA sequence of the donor plants of these two genes were analyzed by PacBio DNA sequencers. DNA sequences of DNA markers used in fine mapping of these two causal genes were used as queries of homology search against DNA sequences derived from the donor plants using software BLAST (NCBI). As a result, a contig of Hwa1-1 was constructed and it had 20 kbp deletion in comparison with the Nipponbare genome sequence. On the other hand, the chromosomal structure of Hwa2-1region had large difference with that of Nipponbare homologous region, and we could not construct a contig covering the Hwa2-1interval.
Type Local :科研等報告書
Publisher :茨城大学
URI :http://hdl.handle.net/10109/14010
Appears in Collections:Grants-in-aid for scientific research Report (College of Agriculture)

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